Lock falls in singles, while Lock and Nunez drop doubles match against Oklahoma State.
The junior fought off two match points to move to the Round of 32.
Two winners selected nationally receive $10,000 for outstanding academic performance.
After an injury derailed their plans in 2014, Benjamin Lock and Marco Nunez are hoping to become All-American's at the NCAA Singles and Doubles Championships.
Junior Benjamin Lock opens singles play Wednesday.
The Seminoles streamed five home matches live on ESPN3 during the season.
Lock wins Arthur Ashe Award, while Crowell is named the Assistant Coach of the Year.
Senior Cristian Gonzalez Mendez ends career winning the only set for the ‘Noles.
Wins from FSU's lone senior and a pair of juniors helped spur win over Troy.
Florida State will face Troy at 11 a.m.; No. 8 Georgia and Winthrop face off at 2 p.m.
Both juniors will play doubles, while Lock will also play in the singles draw.
Florida State will head to the Athens Regional for the third time in six seasons.